Ita is produced from the pyrolysis of citric acid or through the fermentation of carbohydrates forming itaconic acid followed by its dehydration to form the anhydride 3, and sm is derived from renewable plant oils 46. Pdf production of itaconic acid by ustlago maydis from. In general, glucose, sucrose and xylose are preferred raw materials for itaconic acid fermentation, which are known to be utilized efficiently by most of the aspergillus sp. Competitive landscape chinese players dominate the world itaconic acid market table 21. Citric acid, acetic acid, lactic acid, gluconic acid, and itaconic acid are some of the organic acids used widely in various industries. Direct fungal fermentation of lignocellulosic biomass into. Process intensification for separation of carboxylic acids. Study of itaconic acid production by aspergillus terrus mjl05 strain. Production of itaconic acid by ustlago maydis from agro wastes in solid state fermentation. Unsaturated solid bond makes a conjugated system with carbonly group.
The production can be done using submerged solid fermentation, and the typical substrates are. Particularly, it can even replace acrylic and methacrylic acid. An improved process for the production of itaconic acid andor a salt thereof by aerobic microbial fermentation of a nutrient medium containing a source of assimilable carbon and at least one microorganism which produces itaconic acid andor a salt thereof when cultured in the presence of said assimilable carbon, the improvement wherein said source of assimilable carbon at. In this work it was found that the production of itaconic acid is higher in fermentations initiated with lower cellular densities.
Itaconic acid also called methylene succinic acid is a white crystalline carboxylic acid obtained by the. This dicarboxylic acid is a white solid that is soluble in water, ethanol, and acetone. In general, glucose, sucrose and xylose are preferred raw materials for itaconic acid fermentation, which are known. Pdf fungal fermentation of lignocellulosic biomass for. The easy availability of itaconic acid led to its use in the synthesis of menadione. Organic acids are obtained as the endproducts or sometimes as the intermediate components of a particular biochemical cycle. Solid state fermentation product recovery advantages of biobased production 6. Percentage breakdown of volume production for chinese and nonchinese producers includes corresponding graphchart. Jan 09, 2001 itaconic acid solid state fermentation process with peeled sugarcane pressmud as support and starch hydrolysate as carbon source. Since the 1960s it has been produced by fermentation with aspergillus. Itaconic acid is also produced by submerged liquid state fermentation slf but the yield of itaconic acid observed is less in this method. Method for the production of itaconic acid using aspergillus terreus. The itaconic acid produced by liquid state fermentation is 4. The growth of the organisms can be accelerated by the addition of.
Direct production of itaconic acid from liquefied corn starch. The name itaconic acid was devised as an anagram of aconitic acid, another derivative of citric acid. Up to now, the microorganism based processes were improved by classical strain breeding and optimizations of the fermentation strategies and conditions. Pdf biobased itaconic acid market and research trendsis. Itaconic acid production by novel aspergillus niveus in solid state fermentation using agrowastes. Polymers produced according to the process of the invention are useful as detergent additives, scale inhibitors and removers, sequestrants, yarn sizers, deflocculating.
There is a clear distinction between the primary metabolism and product formation in type iii fermentation fig. Synthesis of biobased methacrylic acid by decarboxylation. Itaconic acid ia is one of the most important organic acids that can be. Solidstate fermentation for the production of organic. It has a role as a fungal metabolite and a human metabolite. Itaconic anhydride ita and stearyl methacrylate sm are other monomers obtained from renewable resources. Aspergillus terreus produces itaconic acid at low ph but lovastatin and other secondary metabolites at higher ph in the fermentation. Monosaccharides were obtained by pretreatment and enzymatic hydrolysis of bamboo residues. Itaconic acid methylene succinic acid is produced commercially by microbial fermentation using the fungus a. Itaconic acid market global industry analysis and forecast 2023. These acids are used as building blocks for large numbers of compounds, such as resins, paints, plastics, and synthetic fibers acrylic plastic, super absorbants, and antiscaling agents 67. Cmecooh 3, which was refluxed for 24 h with amalgamated zn prepared from zn and hgcl 2 in concentrated hcl, h 2 o, phme, and acoh, plus 4 cc additional concentrated hcl each 6 h. The market volume of itaconic acid has been estimated to be about 15,000 metric tons per year and is expected to grow if the selling price is reduced. Biobased itaconic acid is expected to find its application in manufacturing adhesives and sealants and is expected to foresee growth on account of development of biobased adhesives and inclination of chemical manufacturers towards.
Polymers of itaconic acid are formed at high conversion by an aqueous polymerization process of partially neutralized monomer solution, water, polyvalent metal ion and initiator. Ia is used in artificial glass, bioactive compounds in pharmacy, medicine, agriculture, for the synthesis of fiber, resin, plastic, rubber, paints, surfactant, ionexchange resins and lubricant. It is a dicarboxylic acid and an olefinic compound. At the end of the process, citric acid can be extracted into hot water and isolated. Hydrochloric acid was chosen to allow comparisons with the initial screening experiments and due to the small amounts needed to change the ph to a value less than p k a. In solidstate fermentation processes, there is lack of free water in the culture medium, with only enough amount to maintain the growth of the microorganism pandey et al. Strikingly, no production of itaconic acid was obtained when a prototrophic strain was used as a host for the production of itaconic acid, indicating that amino acid. Itaconic acid ia production by aspergillus terreus mjl05 strain was investigated in submerged batch fermentation in a stirred bioreactor to determine the effect of varying the nitrogen, phosphorous and carbon sources concentrations in the production medium.
Production of some amino acids, citric acid and itaconic acid are good examples of type ii fermentation. Beside the investigations of individual unit operations, the research on process chains, leading from plant biomass to the final products like lactic acid, succinic acid, and itaconic acid is increasing. Information about the openaccess article production of itaconic acid by ustilago maydis from agro wastes in solid state fermentation in doaj. The utilization of glucose as a carbon substrate was investigated for secondary metabolite production by a.
Name date 11 csource fermentation pilot c6 cellulose labscale c6 cellulose labscale glucose end of fermentation h 115 146 260 itaconic acid produced gl 65. Aspergillus niger was selected as a host for producing itaconic acid due to its versatile and tolerant character in various growth environments, and its extremely high capacity of accumulating the precursor of itaconic acid. Itaconic acid is a dicarboxylic acid that is methacrylic acid in which one of the methyl hydrogens is substituted by a carboxylic acid group. Synthesis of itaconic acid ester analogues via selfaldol. Itaconic acid is an example of a dicarbonic unsaturated acid. Itaconic acid ia is one of the hopeful substances within the cluster of organic acids. Methacrylic acid was synthesized from the biobased substrates citric acid, itaconic acid, and 2hydroxyisobutyric acid 2hiba. Synthesis of methacrylic acid by catalytic decarboxylation. Production of citric acid by submerged fermentation. Since the 1960s the production of itaconic acid is achieved by. Effect of potassium ferrocyanide on the chemical composition of molasses mash used in the citric acid fermentation.
Production of important organic acids by fermentation. These results open the door to the successful exploration of yeast as a suitable microbial cell factory for the production of itaconic acid. In this context, itaconic acid ia or methylene succinic acid 1, fig. World market and biotechnological production of itaconic acid. Although some citric acid is produced by solid state fermentation, the estimated 2. Itaconic acid ia is a dicarboxylic acid included in the us. The bioproduction of highvalue chemicals such as itaconic and fumaric acids ia and fa, respectively from renewable resources via solid state fermentation ssf represents an alternative to the current bioprocesses of submerged fermentation using refined sugars. Production of itaconic acid by fermentation rhonepoulenc. In these processes, the solid material most often used is agroindustrial waste, which serves both as. Hafniumcontaining bea hfbea zeolites catalyze the condensation of ethyl pyruvate into diethyl 2methyl4oxopent2enedioate and diethyl 2methylene4oxopentanedioate an itaconic acid ester analogue with a selectivity of ca.
Pdf filamentous fungi from the genus aspergillus are of high importance for the production of organic acids. Itaconic and fumaric acid production from biomass hydrolysates by. Exploring saccharomyces cerevisiae to improve microbe. Itaconic acid is an unsaturated dicarbonic acid which has a high potential as a biochemical building block, because it can be used as a monomer for the production of a plethora of products including resins, plastics, paints, and synthetic fibers. Itaconic acid a versatile building block for renewable. Enhanced itaconic acid production in aspergillus niger using.
Study of itaconic acid production by aspergillus terrus. Submerged citric acid fermentation of beet molasses in tanktype fermenters. Itaconic acid definition of itaconic acid by the free. Cassava bagasse was found to be a better substrate than vegetable sponge and sugar cane bagasse. The bioproduction of highvalue chemicals such as itaconic and fumaric acids ia and fa, respectively from renewable resources via solidstate fermentation ssf represents an alternative to the current bioprocesses of submerged fermentation using refined sugars. The conditions used here are identical to those used in example 1, except that the glucose10% wv in medium a was replaced by 33% wv starch hydrolysate which contained 30% wv total sugar. Renewable raw materials in sustainable biorefinery processes pose new challenges to the manufacturing routes of platform chemicals. Although most of the processes for lactic acid production are conducted by submerged fermentation, some processes in solid state have been successfully developed table 18. A possible substrate for ia production with two strains of a. Gluconic acid production by aspergillus terreus dowdells. Itaconic acid production from undetoxified enzymatic. Itaconic acid 2methylidenebutanedioic acid is an unsaturated dicarbonic acid.
The method to produce itaconic acid is a solidstate fermentation method. Itaconic acid is on the doe department of energy top 12 list of the biotechnologically produced key building blocks in chemicals production, and is a valuable monomer or comonomer in polymers manufacturing 1,2. Enhanced itaconic acid production in aspergillus niger. Itaconic acid ia production by aspergillus terreus mjl05 strain was investigated in submerged batch fermentation in a stirred bioreactor to determine the effect.
Us3162582a process for the production of itaconic acid. Itaconic acid also called methylene succinic acid is a white crystalline carboxylic acid obtained by the fermentation of carbohydrates. Most of the work was done by submerged fermentation conditions. Itaconic acid solidstate fermentation process with peeled sugarcane pressmud as support and starch hydrolysate as carbon source. With a starting ph of 65, glucose was rapidly metabolized to gluconic acid by the wild. Itaconic acid is exclusively produced this way bafana and pandey 2018. For the presented work, each fermentation broth was filtered and the ph of the solid. Itaconic acid fermentation using activated charcoal. In future, it can even replace methacylic acid which is presently produced by the petrochemical industry. Little attention was given on production of ia by solid state fermentation ssf tsai et al. Development of solid state fermentation is expected to open new avenues for biobased itaconic acid market.
Method for the production of itaconic acid using aspergillus. Hydrotalcite, a solid base catalyst, was employed to form methacrylic acid maa through decarboxylation of itaconic acid and citric acid. Biotechnology and bioengineering 1965, 7 2, 269278. Aug 17, 2014 itaconic acid methylene succinic acid is produced commercially by microbial fermentation using the fungus a. It can be used in industry as monomer or comonomer in the manufacturing of polymers, and is on the doe department of energy top 12 list of the biotechnologically produced building block chemicals 1,2. Glycerol, a biodiesel byproduct was reported as an efficient substrate to achieve high itaconic acid productivities. Production of itaconic acid by ustilago maydis from agro. Oct 11, 2018 renewable raw materials in sustainable biorefinery processes pose new challenges to the manufacturing routes of platform chemicals. Itaconic acid, or methylidenesuccinic acid, is an organic compound. Six strains of aspergillus niger were screened in liquid medium and strain lpb 21 was selected for further studies on three different agroindustrial wastesresidues for production of citric acid by solid state fermentation.
It has a broad application spectrum in the industrial production of resins and is used as a building block for acrylic plastics, acrylate latexes, superabsorbents, and antiscaling agents willke and vorlop, 2001. Biobased itaconic acid market size global industry report. Expressing the cad gene from aspergillus terreus opened the metabolic pathway towards itaconic acid in a. Fungal fermentation of lignocellulosic biomass for itaconic and. Sugarcane pressmud or peeled sugarcane pressmud is the support. The itaconic acid produced by solid state fermentation ssf was 8.
Integrated process development of a reactive extraction. Optimized bioproduction of itaconic and fumaric acids. Surface fermentation using liquid as nutrient medium is the. Sigmaaldrich offers a number of itaconic acid products. The fermentation results showed maximum production of itaconic acid of 20.
Itaconic acid ia is a biobased organic acid that can play a very important. Read direct fungal fermentation of lignocellulosic biomass into itaconic, fumaric, and malic acids. The effect of temperature, catalyst mass, residence time, substrate concentration, and fermentation media, on. The itaconic acid anhydride 1 was converted by the friedelcrafts reaction with c 6 h 6 2 and alcl 3 to bzch. The fungal strain aspergillus terreus m69 purchased from shanghai industrial microbiology institute siim, shanghai, china was used for itaconic acid fermentation. Itaconic acid, atcad1, microbased production of itaconic acid s. Although some citric acid is produced by solidstate fermentation, the estimated 2. In particular dicarboxylic acids, like itaconic acid, can be used as monomers for biopolymers. Pdf synthesis of itaconic acid from agricultural waste. Sandersa methacrylic acid, an important monomer for the plastics industry, was obtained in. Feb 14, 20 itaconic acid as a renewable organic acid is of growing interest for the chemical industry, because of its potential to replace crude oil based products like acrylic acid. Citric acid production on three cellulosic supports in.
Pdf production of itaconic acid by ustlago maydis from agro. Solidstate fermentation for the production of organic acids. Both acids are excellent platform chemicals with a wide range of applications in different market, such as plastics, coating, or. The buffer was confirmed to be an inhibitor, and was. Biomassderived production of itaconic acid as a building. Improving itaconic acid production through genetic.
Date fruit pulp was used as a substrate in solid state fermentation for the production of itaconic acid using naturally occurring fungus. Pdf itaconic acid production by novel aspergillus niveus in solid. Itaconic acid is mostly used in the form of derivatives in several enduser industries. Synthesis and characterization of itaconic anhydride and. The bioprocess with filamentous fungi is usually sensitive to hydromechanical stress in submerged fermentation voll et al. Historically, itaconic acid was obtained by the distillation of citric acid, but currently it is produced by fermentation. Itaconic acid is mostly produced by the fungal fermentation of glucose syrup. Solidstate fermentation takes about 80 to 100 hours for maximal production of citric acid. Biobased itaconic acid market size global industry. Lewis acidic zeolites are used to synthesize unsaturated dicarboxylic acid esters via aldol condensation of keto esters. Itaconic acid fermentation using activated charcoaltreated. Aug 27, 2012 aspergillus niger was selected as a host for producing itaconic acid due to its versatile and tolerant character in various growth environments, and its extremely high capacity of accumulating the precursor of itaconic acid. This article presents a complete process chain from wooden.
Direct production of itaconic acid from liquefied corn. Optimized bioproduction of itaconic and fumaric acids based. Us5336744a process for polymerization of itaconic acid. Itaconic acid ia production by fermentation of undetoxified hydrolysate of bamboo residues by aspergillus terreus was demonstrated. The cac intermediate cisaconitate is enzymatically processed by cisaconitate dehycarboxylase cada to produce itaconic acid 68. Types and stages of fermentation process explained. Metabolic engineering strategies to improve yeast based. In order to promote the biobased economy, the usdepartment of energy doe assigned a top12 of platform chemicals, which include numerous of organic acids.
1356 389 777 1340 1319 716 952 598 554 1341 458 1140 684 189 1269 1304 1009 1434 77 864 345 1534 37 1412 1386 1509 729 914 192 1557 1081 267 655 537 244 194 597 910 599